Original
혈관 내피세포에서 Shear Stress에 의한 MAP Kinase (ERK1/2, p38) 활성에 PECAM-1 (CD31)의 역할 기계적 수용기로서의 PECAM-1의 역할
MAP Kinase (ERK1/2, p38) can be Phosphorylated by Shear Stress Independent of PECAM-1 (CD31) in Vascular Endothelial Cells PECAM-1 as a Mechanoreceptor
Korean J Vasc Endovasc Surg 2005; 21(1): 1-9
Published May 30, 2005
Copyright © The Korean Society for Vascular Surgery.
Purpose: Mechanical forces, including shear stress (SS), trigger signal transducing events and modulate gene expression in vascular endothelial cells (ECs). However, the primary steps of mechanoreception are still unknown. PECAM-1 (CD31), a member of the immunoglobulin (Ig) superfamily of cell adhesion molecules, is localized to the interendothelial cell adhesion site. PECAM-1 tyrosine phosphorylation has been observed following mechanical stimulation, but the role it plays in mechanosensing in ECs is controversial. The aim of this study was to confirm the involvement of PECAM-1 in ECs signaling cascades in response to SS. Method: In this study, PECAM-1 knock-out (KO) and wild-type (WT) rat microvascular ECs, and 50 and 100% confluent bovine aortic ECs (BAECs) were exposed to oscillatory SS (14 dyne/cm2) for 0, 5, 10, 30 or 60 minutes. Activation of the PECAM-1 and the mitogen activated protein (MAP) kinase were assessed by determining the phosphorylation of PECAM-1, extracellular signal-regulated kinase1/2 (ERK1/2) and p38 using immunoprecipitation and immunoblotting. Result: The tyrosine-phosphorylation level of PECAM-1 immunoprecipitated from SS-stimulated WT ECs increased. While PECAM-1 was phosphorylated in 100% confluent BAECs, its phosphorylation level in 50% confluent BAECs was not detected by SS. However, ERK1/2 and p38 were also activated by SS in both PECAM-1 KO ECs and 50% confluent BAECs. Conclusion: Our results indicate that ERK1/2 and p38 were activated by SS in PECAM-1 KO ECs and 50% confluent BAECs despite no PECAM-1 phosphorylation. This suggests that PECAM-1 can not be a major mechanoreceptor for the activation of MAP kinase in ECs; therefore, other more important mechanoreceptors maybe present in ECs to detect SS and trigger intercellular signal transduction.
Keywords
Shear stress, MAP Kinases, PECAM-1, Endothelial cell