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Different Effects of Orbital Shear Stress on Vascular Endothelial Cells: Comparison with the Results of In Vivo Study with Rats
Vascular Specialist International 2015;31:33-40
Published online June 30, 2015;
© 2015 The Korean Society for Vascular Surgery.

Hyosoo Kim1, Keun Ho Yang1, Hyunjin Cho1, Geumhee Gwak1, Sun Cheol Park2, Ji Il Kim2, Sang Seob Yun2, and In Sung Moon2

1Department of Surgery, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea, 2Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea
Correspondence to: Keun Ho Yang, Department of Surgery, Sanggye Paik Hospital, Inje University College of Medicine, 1342 Dongil-ro, Nowon-gu, Seoul 139-707, Korea, Tel: 82-2-950-1017, Fax: 82-2-933-9389, E-mail:
Received March 2, 2015; Accepted March 23, 2015.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


An attempt was made to characterize the orbital shear stress by comparing the effects of orbital shear stress on vascular endothelial cells (ECs) with the results of animal experiments.

Materials and Methods:

In the laboratory study, cultured ECs of well were distinguished by center and periphery then exposed to orbital shear stress using an orbital shaker. In the animal study, arteriovenous (AV) fistulas were made at the right femoral arteries of Sprague-Dawley rats to increase the effect of the laminar flow. The condition of the stenosis was given on the left femoral arteries. The protein expression of inducible nitric oxide synthase (iNOS) and Akt phosphorylation were observed and compared.


Under orbital shear stress, ECs showed an increase in iNOS protein expression and phosphorylation of Akt but most of the protein expressions derived from the periphery. When compared to the animal study, the increased expression of iNOS protein and phosphorylation of Akt were observed in the sample of AV fistula conditions and the iNOS protein expression was decreased in the stenosis conditions.


Orbital shear stress did not show the characteristics of a pure turbulent shear force. By comparing the observation with the morphological changes of vascular ECs and site-specific protein expression on the results of animal experiments, uniform directional lamina shear stress forces were expressed at the periphery.

Keywords: Orbital shear stress, Endothelial cells, Akt phosphorylation, Nitric oxide synthase

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